Alkaline Phosphatase (ALP) assay kit

  • For quantitative determination of alkaline phosphatase (ALP) activity
  • Detection limit up to 800 U/L
  • Simple “mix-and-measure” ALP assay; no pre-treatment needed
  • Results within 5 minutes
  • Robust and amenable to HTS

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate.

This colorimetric Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

All reagents are compatible with high-throughput liquid handling instruments.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0013 Alkaline Phosphatase Assay Kit (Colorimetric) - 250 assays
RG BA0169 ALP Detection Reagent - 600 assays
RG BA0170 Alkaline Phosphatase Assay Kit (Fluorometric) - 100 assays