Alkaline Phosphatase (ALP) assay kit
- For quantitative determination of alkaline phosphatase (ALP) activity
- Detection limit up to 800 U/L
- Simple “mix-and-measure” ALP assay; no pre-treatment needed
- Results within 5 minutes
- Robust and amenable to HTS
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate.
This colorimetric Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
All reagents are compatible with high-throughput liquid handling instruments.