Beta-N-acetylglucosaminidase activity assay kit
- For beta-N-acetylglucosaminidase activity determination in biological samples
- Sensitive: linear detection range 0.2 to 50 U/L
- Fast 30 minutes assay
- Convenient “mix-incubate-measure” type assay
- High-throughput: suitable for HTS liquid handling systems
Beta-N-acetylglucosaminidase is a lysosomal enzyme involved in a variety of biological processes such as the degradation of glycoproteins and glycolipids, cell proliferation, and signal transduction. NAG is found in many tissues in the body, but due to its high molecular weight, it can not be filtered through the glomerular membrane.
Our non-radioactive, colorimetric beta-N-Acetylglucosaminidase activity assay kit is based on the cleavage of p-nitrophenol from a synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.