Diamine Oxidase assay kit

  • For quantitative determination of diamine oxidase in biological samples
  • Fast and sensitive: linear detection range 0.5 to 6 U/L
  • Convenient: “Add-mix-read” type assay
  • High-throughput: measure thousands of samples per day

Diamine oxidase is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals.

This non-radioactive, fluorimetric Diamine Oxidase Assay (DAO) assay is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at Ex/Em = 530/585 nm is directly proportional to the enzyme activity.

  • Use of 10 μL sample
  • Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C
  • The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed
  • For serum, plasma samples

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0172 Diamine Oxidase Activity Assay Kit (Fluorometric) - 100 assays