Oxaloacetate assay kit
- For quantitative determination of oxaloacetate
- Sensitive and accurate
- Plasma, serum, tissue and culture media samples
- Results within 20 minutes
Oxaloacetate is an intermediate in the citric acid cycle and participates in gluconeogenesis. It is formed by the oxidation of malate, by deamidation of aspartate or by condensation of CO2 with pyruvate or phosphoenolpyruvate.
In this Oxaloacetate Assay Kit, oxaloacetate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the oxaloacetate concentration in the sample.
- Linear detection range: 7 to 400 μM oxaloacetate for colorimetric assays and 1 to 40 μM for fluorimetric assays.