pNPP Phosphatase assay kit

  • For quantitative determination of phosphatase activity
  • Detection limit <3 ng phosphatase
  • Fast & simple: results within 30 minutes
  • Convenient: “mix-and-measure” type assay
  • Amenable to HTS screening

p-nitrophenyl phosphate (pNPP) is a chromogenic substrate for most phosphatases such as alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

The reaction in this pNPP phosphatase assay kit yields para-nitrophenol, which becomes an intense yellow soluble product under alkaline conditions and can be conveniently measured at 405 nm on a spectrophotometer.

  • Simply add a single reagent to the phosphatase and measure the product formation on an absorbance reader
  • The assay can be conveniently performed in cuvettes, tubes or multi-well plates
  • Eecution at either room temperature or 37°C
  • All reagents are compatible with high-throughput liquid handling instruments.

 pNPP phosphatase activity pNPP phosphatase activity

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Assay Genie website to find your local distributor.
Cat no. Description
RG BA0166 pNPP Phosphatase Assay Kit - 1000 assays
RG BA0167 pNPP Phosphatase Assay Kit - 500 assays