LUNA-FL Automated Fluorescence Cell Counter

Automated cell counting in bright field or fluorescence

The LUNA-FL is an automated cell counter that combines the precision bright field microscopy optics of the Luna and dual fluorescence optics. With the integrated bright field microscopy of the LUNA, the LUNA-FL provides the convenient features of trypan blue stained cell counting for accurate live/dead analysis.

The integrated dual fluorescence optics allow you to get more sensitive cell analysis results, using fluorescently stained cells.

You can count your cell suspensions from concentrations ranging from 50,000 to 10 million cells/mL, with cell sizes ranging from 1 to 90 μm. Various cell types including cell lines, primary cells, stem cells and PBMC's have succesfully been counted with the LUNA-FL.

GFP transfection efficiency analysis

With the LUNA-FL you can evaluate the transfection efficiency of GFP transfected cells within 30 sec. Bright field and fluorescence cell images are analyzed to detect GFP positive and negative cells in your sample and the corresponding fluorescence intensity histogram is displayed on the touch screen for further analysis.

Interactive software tools

After counting, you can immediately monitor which cells are counted as live, in green, or dead, in red, on the LUNA-FL cell counter screen.

You can gate your counted cells based on their size information provided by the LUNA-FL. Using the histogram that displays live and dead cell population, you can easily exclude or include the particles by simple dragging.

Finally, you can easily merge the analyzed cell images from each channel (bright field, green, and red channel) directly on the screen. The brightness of each color can be adjusted for accurate monitoring.

Lower counting cost per sample

To count your cell suspension with the Luna-FL cell counter, innovative precision cell counting slides have been developed that are more efficiently than counting slides that are used with other cell counters. The unit price of these PhotonSlides is almost half of the other brands, saving significant consumable costs.

Data report and printer options

After counting your cells, results are automatically saved in the memory, that can store up to 1,000 counts. If you would like to analyze your results further, results can be exported via the USB port as a .CSV file. You can also save your results and images  as a PDF report file.

If you would like to print your data immediately  after getting your counting results, the LUNA-FL provides a compact mobile printer. This plug & play mobile printer is ready-to-use after the USB to USB connection and accepts thermal roll paper or label paper.