Insect-XPRESS Protein-free Medium
- For sf9, sf21, High Five™ and Drosophila cells
- Protein-free and serum-free
- For suspension and adherent cells
- For baculovirus propagation and recombinant protein expression
Insect-XPRESS Medium is designed to support the growth of invertebrate cell lines derived from the fall army worm, Spodoptera frugiperda. The medium can be used for suspension, monolayer and shakeflask cultures of sf9, sf21, High Five™ and Drosophila cells.
This protein-free medium contains L-glutamine and supports superior production of recombinant proteins by cells infected with baculoviral vectors.
Most insect cell lines do not require weaning from serum-containing or serum-free media into Insect- XPRESS™ Medium. If adaptation is required, we recommend an initial split ratio of 1:2 into Insect-XPRESS™ Medium followed by sequential splits of 1:5 until the culture is adapted to the new medium. During the weaning process, the cell concentration should be maintained above 3.0 x 105 cells/ml.