HL-1 FBS Substitute

  • Replacement for serum
  • No more batch testing
  • No risk of pathogens or other contaminants
  • Low protein level: <30µg/ml
  • Supports growth of variety of cell lines

Serum replacement

HL-1 FBS Substitute is a medium additive that can be used to (partially) replace serum in a variety of basal media. It contains less than 30 μg protein/ml when diluted 1:100 in medium and it does not contain bovine serum albumin or other undefined protein components.

Growth support of numerous cell lines

HL-1 serum replacement, when added to most basal media, will support the growth of mouse hybridoma cells and other differentiated cells of lymphoid origin (e.g. U937, MCF-7, SW780 and many more). 

Download the full list of tested cell lines.

Suitable media include RPMI 1640, IMDM and DMEM:F12. In addtion, for serum-reduced cell culture DMEM containing 1% FBS can be used.

Adaptation of serum-dependent cells to medium with HL-1

Weaning cell lines from serum-containing media may be recommended. The following protocol will aid adaptation to a serum-free environment in media containing HL-1™ Supplement by gradual reduction of the serum concentration:

  • Week 1 – Reduce the serum concentration to 5% FBS.
  • Week 2 – Reduce the serum concentration to 2% FBS and add HL-1™ Supplement at 1%.
  • Week 3 – Reduce the serum concentration to 1% FBS with HL-1™ Supplement at 1%.
  • Week 4 – Reduce the serum concentration to 0.5% FBS with HL-1™ Supplement at 1%.
  • Week 5 – Reduce the serum concentration to 0.25% FBS with HL-1™ Supplement at 1%.
  • Week 6 – Eliminate FBS and culture cells in serum-free HL-1™ Supplement-containing medium.

NOTE: At each step in the weaning process, cells will show an initial lag in growth rate. Passage the cells three times per week during the weaning period, seeding at a density of 1-2 x 105 cells/ml. Keep cell density below 8-10 x 105 cells/ml. After reduction to 0.5% serum, a greater growth lag may be observed. Under these conditions, a higher seeding density and less frequent passaging may be required until cells resume their normal growth characteristics.


Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Lonza website to find your local distributor.
Cat no. Description
LO 77227 HL-1 Chemically Defined FBS Substitute 10 ml