UltraMEM Reduced Serum Medium
- Chemically defined medium with reduced serum concentrations
- Weaning of cells unnecessary
- Low protein concentration of human insulin and transferrin (20 μg/ml)
UltraMEM™ Reduced Serum Media are chemically defined media designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations.
Good cell growth performance in reduced serum media
When supplemented with 2 – 4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages. In addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all.
Low protein concentrations
Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, selenium, and ethanolamine (ITS or ITES) to the basal medium. UltraMEM™ Reduced Serum Media are offered as a protein-free basal medium without L-glutamine, or as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/ml.