UltraMEM Reduced Serum Medium
- Chemically defined medium with reduced serum concentrations
- Weaning of cells unnecessary
- Low protein concentration of human insulin and transferrin (20 μg/ml)
UltraMEM™ Reduced Serum Media are chemically defined media designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations.
Good cell growth performance in reduced serum media
When supplemented with 2 – 4% serum, UltraMEM™ Media growth performance is comparable and, in some instances, exceeds that of standard media supplemented with 10% fetal bovine serum. Weaning is not necessary for most applications. Further reduction in serum concentration (<2%) can be achieved over several passages. In addition, confluent cultures can be maintained with minimal amounts of serum (≤1%) or no serum at all.
Low protein concentrations
Growth performance in UltraMEM™ Media can be further increased by the addition of insulin, transferrin, selenium, and ethanolamine (ITS or ITES) to the basal medium. UltraMEM™ Reduced Serum Media are offered as a protein-free basal medium without L-glutamine, or as a complete low protein medium supplemented with ITES. Recombinant human insulin and transferrin are the only protein components of the complete formulation and are present at a total concentration of 20 μg/ml.
Want to find out how you can start with serum reduced media?
Do you wonder how you can get started with serum reduced media for your cells? Let us guide you through the steps of switching to serum-free media!