X-VIVO Serum-free, Chemically Defined Hematopoietic Cell Media

  • Serum-free media and chemically defined
  • For proliferation of peripheral blood lymphocytes or tumor infiltrating lymphocytes
  • For cryopreservation of human tissue
  • For cultivation of human monocytes, macrophages, stem- and dendritic cells

Serum-free media for optimal growth of hematopoietic cells

X-VIVO™ are serum-free and chemically defined hematopoietic cell media. They provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).

Complete and defined media formulations

The X-VIVO™ serum-free media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are free of any protein kinase C stimulators and can be used for studying activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and pasteurized human transferrin.

X-VIVO™ serum-free media variants

X-VIVO™ 10 culture medium formulations support the generation of lymphokine activated killer cells (LAK cells) in a serum-free environment.

X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media but are optimized for the serum-free proliferation of tumor infiltrating lymphocytes (TIL). X-VIVO™ 15 supports proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells.

X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte-depleted peripheral blood lymphocytes (PBL) at high density. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

All X-VIVO™ Media products are manufactured under current GMPs and are listed with the FDA in a product Master File.

Note: catalog numbers LO BE04-418F, LO BE04-418Q and LO BE04-744Q are discontinued and replaced by catalog numbers LO BE02-053Q and LO BE02-054Q.

Need help with your serum-free switch?

Do you need any help with your switch to X-VIVO™ serum-free media? Let us guide you through the steps of switching to serum-free media!

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Lonza website to find your local distributor.
Cat no. Description
LO BE02-053Q X-VIVO 15 with Gentamicin, Phenol Red and Transferrin,1L
LO BE02-054Q X-VIVO 15 without Gentamicin or Phenol Red, with Transferrin,1L
LO BE02-055Q X-VIVO 10 without Gentamicin, Phenol Red, with Transferrin, 1L
LO BE02-060Q X-VIVO 15 with Gentamicin and Phenol Red, 1 L
LO BE02-061Q X-VIVO 15 without Gentamicin and without Phenol Red, 1 L
LO BE04-380Q X-VIVO 10 with Gentamicin and Phenol Red, 1 L
LO BE04-448Q X-VIVO 20 with Gentamicin and Phenol Red, 1 L
LO BE04-743Q X-VIVO 10 without Gentamicin or Phenol Red, 1 L