X-VIVO Serum-free Media for immune cells

  • Serum-free media
  • For proliferation of peripheral blood lymphocytes or tumor infiltrating lymphocytes
  • For cryopreservation of human tissue
  • For cultivation of various immune cells

Serum-free media for optimal growth of immune cells

X-VIVO media are serum-free cell culture media to cultivate immune cells, amongst others hematopoietic cells. They provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).

Serum-free X-VIVO media outperforms serum-based media in 6-well plates (left) and a closed system breathable vessel (right). Click to enlarge. Serum-free X-VIVO media outperforms serum-based media in 6-well plates (left) and a closed system breathable vessel (right). Click to enlarge.

Complete and defined media formulations

The X-VIVO serum-free media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are free of any protein kinase C stimulators and can be used for studying activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and pasteurized human transferrin.

X-VIVO serum-free media variants

X-VIVO 10 culture medium formulations support the generation of lymphokine activated killer cells (LAK cells) in a serum-free environment.

X-VIVO 15 Media are similar in composition to X-VIVO™ 10 Media but are optimized for the serum-free proliferation of tumor infiltrating lymphocytes (TIL). X-VIVO™ 15 supports proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells.

X-VIVO 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte-depleted peripheral blood lymphocytes (PBL) at high density. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

All X-VIVO Media products are manufactured under current GMPs and are listed with the FDA in a product Master File.

Changes to X-VIVO™ Media without gentamicin and phenol red

  • Going forward several Lonza products currently branded "X-VIVO™ Media" will be branded "TheraPEAK™ X-VIVO™ Media"
  • These products with the current label claim "Research Use Only" (RUO) will in future be labelled "For Further Manufacturing" (FFM) and will be produced according to ISO 13485:2016.
  • "Research Use Only" (RUO) labelled products will be available until current stock is depleted.
  • Media labelled "For Further Manufacturing" will have a different catalog number.
  • Formulation and pricing will remain the same

RUO (Research Use Only) Labelled Item

FFM (For Further Manufacturing) Labelled Item

LO 04-744Q
X-VIVO 15 w/o Gentamicin and w/o Phenol Red, 1 L

LO BP04-744Q
X-VIVO 15 w/o Gentamycin and Phenol Red, FFM, 1 L

LO BE02-054Q (discontinued)
X-VIVO 15 w/o Gentamicin or Phenol Red, with Transferrin,1L

LO BEBP02-054Q
TheraPeak X-VIVO 15 with Recombinant Transferrin, w/o Gentamicin or Phenol Red 1L

LO BE02-055Q (discontinued)
X-VIVO 10 w/o Gentamicin, Phenol Red, with Transferrin, 1L

LO BEBP02-055Q
Therapeak X-VIVO 10 with Recombinant Transferrin, w/o Gentamicin or Phenol Red, 1L

LO BE02-061Q
X-VIVO 15 w/o Gentamicin and w/o Phenol Red, 1 L

LO BEBP02-061Q
TheraPeak X-VIVO 15, w/o Gentamicin or Phenol Red, 1L

LO BE04-743Q
X-VIVO 10 w/o Gentamicin or Phenol Red, 1 L

LO BEBP04-743Q
X-VIVO 10, w/o Gentamicin or Phenol Red, FFM, 1L

LO BE02-053Q
X-VIVO 15 with Gentamicin, Phenol Red and Transferrin,1L

no change

LO BE02-060F
X-VIVO 15 with Gentamicin and Phenol Red, 500 ml

no change

LO BE02-060Q
X-VIVO 15 with Gentamicin and Phenol Red, 1 L

no change

LO BE04-380Q
X-VIVO 10 with Gentamicin and Phenol Red, 1 L

no change

LO BE04-448Q
X-VIVO 20 with Gentamicin and Phenol Red, 1 L

no change

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Lonza website to find your local distributor.
Cat no. Description
LO BE02-053Q X-VIVO 15 with Gentamicin, Phenol Red and Transferrin,1L
LO BE02-060F X-VIVO 15 with Gentamicin and Phenol Red, 500 ml
LO BE02-060Q X-VIVO 15 with Gentamicin and Phenol Red, 1 L
LO BE04-380Q X-VIVO 10 with Gentamicin and Phenol Red, 1 L
LO BE04-448Q X-VIVO 20 with Gentamicin and Phenol Red, 1 L
LO BE04-743Q X-VIVO 10 without Gentamicin or Phenol Red, 1 L
LO BEBP02-054Q TheraPeak X-VIVO 15 with Recombinant Transferrin, without Gentamicin or Phenol Red, FFM, 1L
LO BEBP02-055Q TheraPeak X-VIVO 10 with Recombinant Transferrin, without Gentamicin or Phenol Red, FFM, 1L
LO BEBP02-061Q TheraPeak X-VIVO 15, without Gentamicin or Phenol Red, FFM, 1L