X-VIVO Serum-free Media for immune cells
- Serum-free chemically defined media
- For proliferation of peripheral blood lymphocytes or tumor infiltrating lymphocytes
- For cryopreservation of human tissue
- For cultivation of various immune cells
Serum-free media for optimal growth of immune cells
X-VIVO media are serum-free and chemically defined cell culture media to cultivate immune cells, amongst others hematopoietic cells. They provide nutritionally complete and balanced environments for a variety of cells including lymphokine activated killer (LAK) cells, peripheral blood lymphocytes (PBL), and tumor infiltrating lymphocytes (TIL).
Complete and defined media formulations
The X-VIVO™ serum-free media do not contain any exogenous growth factors, artificial stimulators of cellular proliferation, or undefined supplements. They are free of any protein kinase C stimulators and can be used for studying activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin, and pasteurized human transferrin.
X-VIVO™ serum-free media variants
X-VIVO™ 10 culture medium formulations support the generation of lymphokine activated killer cells (LAK cells) in a serum-free environment.
X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media but are optimized for the serum-free proliferation of tumor infiltrating lymphocytes (TIL). X-VIVO™ 15 supports proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells.
X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocyte-depleted peripheral blood lymphocytes (PBL) at high density. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).
All X-VIVO™ Media products are manufactured under current GMPs and are listed with the FDA in a product Master File.
Note: catalog numbers LO BE04-418F, LO BE04-418Q and LO BE04-744Q are discontinued and replaced by catalog numbers LO BE02-053Q and LO BE02-054Q.
Need help with your serum-free switch?
Do you need any help with your switch to X-VIVO™ serum-free media? Let us guide you through the steps of switching to serum-free media!