X-CLARITY™ Tissue Clearing System

  • All-in-one, easy-to-use solution for electrophoretic tissue clearing
  • Exceptional transparency reached, even for hard tissues like bone
  • Pre-optimized protocols
  • Prepared tissues ready for efficient antibody labeling and imaging

X-CLARITY Tissue Clearing System Year-End Offer

Enjoy our year-end offer: order an X-CLARITY Tissue Clearing System before Dec. 15th, 2017 and get:

  • Free reagent pack incl. ETC solution (12L) + Mounting solution (10x25mL) + Hydrogel (1L)
  • When combined with purchase of the X-CLARITY™ Polymerization System you'll get 15% discount on the polymerization system plus the free reagent pack.

Scroll down to get your personalized quote.

Spend your time more efficiently and decrease your time spent on preparing, sectioning, labeling, mounting, imaging and analyzing those hundreds of ultra-thin slices when you want to analyze tissues. X-CLARITY™ offers you the ultimate solution for electrophoretic tissue clearing: prepare your tissue in its whole for further histology and imaging analysis.

Tissue clearing with X-CLARITY™: how it works

With X-CLARITY™ you clear whole tissues from their lipid bilayers (through electrophoresis in the presence of ionic detergents) while keeping the biomolecules intact, which are covalently linked to a sturdy hydrogel network.

This tissue clearing method preserves the molecular information and structural integrity of a tissue sample and leaves you with a stable and transparant tissue-hydrogel hybrid. This hybrid can be efficiently penetrated by and labeled with antibodies or oligonucleotides for further phenotyping.

Mouse tissues cleared with the X-CLARITY™ Tissue Clearing system Mouse tissues cleared with the X-CLARITY™ Tissue Clearing system

Tissue clearing workflow

The superfast and efficient workflow of X-CLARITY™ tissue clearing involves four steps:

  1. Incubation of the tissue sample in a hydrogel monomer solution to allow uniform hydrogel diffusion throughout the whole sample
  2. Polymerization to form the stable hydrogel-tissue hybrid
  3. Clearing of the lipid bilayers in the tissue-hydrogel hybrid through electrophoresis in the presence of ionic detergents
  4. Labeling and imaging of your whole tissue
Mouse brain cleared with X-CLARITY™ and labeled with anti-tyrosine hydroxylase Mouse brain cleared with X-CLARITY™ and labeled with anti-tyrosine hydroxylase
Thy1-YFP Transgenic mouse brain cleared with X-CLARITY™ ; neurons (green), ECM (red) and nuclei (blue) are labeled Thy1-YFP Transgenic mouse brain cleared with X-CLARITY™ ; neurons (green), ECM (red) and nuclei (blue) are labeled
 
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Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Logos Biosystems website to find your local distributor.
Cat no. Description
LB C10001 X-CLARITY Tissue Clearing System Starter Kit
LB C12001 Tissue Container (Whole Mouse Brain) - 20 units
LB C12002 Container Holder for 1 Tissue Container
LB C12004 Mouse Brain Slice Holder
LB C12007 Whole Rat Brain Holder
LB C13001 Electrophoretic Tissue Clearing Solution (12x1L)
LB C13100 X-CLARITY Mounting Solution (5x1ml)
LB C13101 X-CLARITY Mounting Solution (25ml)
LB C13102 X-CLARITY Mounting Solution (Value Pack) (10x25ml)