MHC I-Strep Reagents

  • Isolate and characterize fully functional antigen-specific T-cells
  • For fluorescent staining/FACS sorting and magnetic isolation

The Streptamer technology is a novel method to isolate and characterize fully functional antigen-specific (CD8+) T-cells. The technology is based on a reversible staining that leaves you with unaltered T-cells.

MHC I-Strep reagents are either combined with fluorescence- or bead-labeled Strep-Tactin® for cell staining or magnetic isolation, respectively.

T-cell isolation with streptamers: the principle

The MHC I-Strep reagents are used in combination with Strep-Tactin® Magnetic Beads to isolate antigen-specific (CD8+) T-cells from PBMCs, using magnetic columns. The MHC I-Strep reagents can also be used for FAC analysis/sorting when they are combined with a Strep-Tactin® fluorochrome. The principle of how they work is similar:

  • The antigen-loaded MHC I-Strep reagent contains the Strep-tag® affinity tag
  • The magnetic beads or the fluorochromes are covalently linked to Strep-Tactin®
  • The MHC I-Strep reagent binds the T-cells of interest to its antigen on one side, and binds the Strep-Tactin® Magnetic Bead or Strep-Tactin® fluorochrome on the other side of its molecule. This way, the T-cells of interest can be selected by magnetic sorting columns or fluorochrome excitation on FACS or by fluorescent staining, respectively.
  • After isolation/sorting of the T-cells, D-biotin is added to the suspension that releases the Strep-Tactin® Magnetic Bead or Strep-Tactin® fluorochrome.
  • Subsequently, the MHC I-Strep reagent will spontaneously dissociate due to its weak binding.

 

Ordering information

Westburg offers this product in The Netherlands, Belgium, Finland, Luxembourg, Denmark, Sweden, Norway, Iceland,
If you are not located in these countries please visit the IBA website to find your local distributor.
Cat no. Description
IB 6-7001-005 MHC I-Strep HLA-A*0201 CMV PP65, NLVPMVATV 50
IB 6-7001-901 CMV pp65 peptide NLVPMVATV (HLA-A 0201) for stimulation of T-Cells, 1mg
IB 6-7025-901 gp100 peptide YLEPGPVTA for stimulation of T-cells, 1mg
IB 6-7027-901 CMV pp65 peptide TPRVTGGGAM (HLA-B*0702) for stimulation of T-Cells, 1mg