MHC I-Strep Reagents
- Isolate and characterize fully functional antigen-specific T-cells
- For fluorescent staining/FACS sorting and magnetic isolation
The Streptamer technology is a novel method to isolate and characterize fully functional antigen-specific (CD8+) T-cells. The technology is based on a reversible staining that leaves you with unaltered T-cells.
T-cell isolation with streptamers: the principle
The MHC I-Strep reagents are used in combination with Strep-Tactin® Magnetic Beads to isolate antigen-specific (CD8+) T-cells from PBMCs, using magnetic columns. The MHC I-Strep reagents can also be used for FAC analysis/sorting when they are combined with a Strep-Tactin® fluorochrome. The principle of how they work is similar:
- The antigen-loaded MHC I-Strep reagent contains the Strep-tag® affinity tag
- The magnetic beads or the fluorochromes are covalently linked to Strep-Tactin®
- The MHC I-Strep reagent binds the T-cells of interest to its antigen on one side, and binds the Strep-Tactin® Magnetic Bead or Strep-Tactin® fluorochrome on the other side of its molecule. This way, the T-cells of interest can be selected by magnetic sorting columns or fluorochrome excitation on FACS or by fluorescent staining, respectively.
- After isolation/sorting of the T-cells, D-biotin is added to the suspension that releases the Strep-Tactin® Magnetic Bead or Strep-Tactin® fluorochrome.
- Subsequently, the MHC I-Strep reagent will spontaneously dissociate due to its weak binding.