Strep-Tactin PE/APC

  • Strep-Tactin with bound fluorochrome
  • For T-cell staining and isolation using FACS

T-cell staining and FACS sorting with the Streptamer technology

The Streptamer technology is a novel method to isolate and characterize functional antigen-specific (CD8+) T-cells.

With the Strep-Tactin® PE or APC, you can quickly stain and sort antigen-specific (CD8+) T-cells without phenotypic and functional change of your T-cells. This T-cell isolation technology is based on a reversible staining.

Fluorescent staining of T-cells with Streptamers: how it works

Strep-Tactin PE and Strep-Tactin APC are suitable for the staining of antigen-specific (CD8+) T-cells when combined with MHC I-Strep reagents.

  • Prior to staining of your T-cells, a pre-incubation step is required to associate the MHC I-Strep reagents with the Strep-Tactin® PE or -APC.
  • The resulting Streptamer® multimers are then used for antigen-specific staining of your targeted T-cells.
  • After staining and FACS sorting, the Strep-Tactin® PE or -APC can be completely removed from the cells by addition of biotin.
  • Ultimately, the Strep-tagged MHC I-Strep reagents are then spontaneously dissociated from the sorted T-cells, due to its weak binding.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Finland, Luxembourg, Denmark, Sweden, Norway, Iceland,
If you are not located in these countries please visit the IBA website to find your local distributor.