Streptamer Magnetic Beads

  • For rapid and economic (CD8+) T-cell isolation
  • Reversible, antigen-specific staining
  • Mild and rapid dissociation using biotin
  • For magnetic isolation

T-cell isolation with the Streptamer technology

The Streptamer technology is a novel method to isolate and characterize functional antigen-specific (CD8+) T-cells. With the Strep-Tactin® Magnetic Beads, you can quickly isolate antigen-specific (CD8+) T-cells without phenotypic and functional change of your T-cells. This T-cell isolation technology is based on a reversible staining.

The Strep-Tactin® Magnetic Beads are polysaccharide coated magnetic microspheres that are covalently linked to Strep-Tactin. The beads are designed to be used for T-cell isolation with Milteny columns/machines at 4 °C under sterile conditions.

Magnetic isolation of T-cells: the principle

For the isolation of antigen-specific CD8+ T-cells, Strep-Tactin® Magnetic Beads have to be combined with MHC I Strep Reagents (antigen-loaded MHC I containing the Strep-tag® affinity tag).

  • After binding of the MHC I strep reagent of choice, the magnetic bead binds to the tagged T-cells of interest.
  • Using a magnetic column, the labeled T-cells are separated from other cells and eluted from the column by a washing step.
  • By addition of D-biotin to the isolated cell suspension, the Strep-Tactin® Magnetic Beads can be mildly dissociated from the MHC I-Strep reagent.
  • The binding of the MHC I strep reagent to the T-cells is very weak and spontaneously dissociates hereafter, leaving you with a functional and label-free antigen-specific (CD8+) T-cell of interest.

Cat. nr IB 6-5500-005 is sufficient to isolate T-cells out of a total of 1x108 cells.

The Solution Set includes Buffer IS for washing and Biotin for dissociation.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Finland, Luxembourg, Denmark, Sweden, Norway, Iceland,
If you are not located in these countries please visit the IBA website to find your local distributor.
Cat no. Description
IB 6-5500-005 Streptamer Magnetic Beads (5)