jetCRISPR RNP transfection reagent

  • RNP transfection reagent for CRISPR-Cas9 genome editing
  • Specifically designed for Cas9 protein and guide RNA delivery
  • High genome editing efficiency in a wide variety of cell types
  • Excellent cell viability and morphology
  • Compatible with serum and antibiotics

CRISPR/Cas9 is a powerful and highly specific genome editing tool. Choosing the correct method to deliver both Cas9 and guide RNA to your target cells is critical for CRISPR-Cas9 genome editing, as inadequate expression of either component will result in failed genome editing.

jetCRISPR is a RiboNucleoProtein (RNP) transfection reagent that has been specifically designed for efficient co-delivery of Cas9 protein and guide RNA in a wide variety of target cell types.

Request your sample below!

High CRISPR-Cas9 genome editing efficiency

jetCRISPR™ has been specifically designed for high transfection efficiency of RNP (guide RNA and Cas9 protein complex), thus leading to a high cas9-mediated genome editing efficiency in a variety of adherent and suspension cell types. With jetCRISPR™ you can obtain superior genome editing efficiency in comparison with Lipofectamine® CRISPRMAX™.

Superior genome editing efficiency with jetCRISPR™ in comparison with Lipofectamine® CRISPRMAX™ Superior genome editing efficiency with jetCRISPR™ in comparison with Lipofectamine® CRISPRMAX™

Combine jetCRISPR with our optimized Cas9 protein, SpCas9 Nuclease, to improve your CRISPR-Cas9 genome editing efficiency even further. SpCas9 Nuclease is a recombinant S. pyogenes Cas9 nuclease that has been optimized to improve nuclear entry following jetCRISPR™-mediated intracellular delivery. The sequence of spCas9 nulcease contains a single SV40 nuclear localization sequence (NLS) in N- and C-terminus.

Higher genome editing obtained with SpCas9 Nuclease when compared to other Cas9 proteins. Higher genome editing obtained with SpCas9 Nuclease when compared to other Cas9 proteins.

Excellent cell viability and morphology after jetCRISPR RNP transfection

The jetCRISPR RNP transfection reagent is extremely gentle to cells, maintaining an excellent cell viability and morphology throughout the whole duration of your CRISPR-Cas9 genome editing experiments. jetCRISPR™ is compatible with both serum and antibiotics so you can perform your RNP transfection in the optimal condition suitable for your cells.

Fast and reliable CRISPR-Cas9 genome editing

Delivering the Cas9 protein to your cells rather than the Cas9 plasmid allows for faster genome editing: the protein is readily available for use. The Cas9 protein will be cleared from the cells faster than the plasmid, hence minimizing off-target effects and leading to more specific genome editing (Kim S, et al; Genome Research 2014; 24:1012–1019).

Using jetCRISPR™ to deliver Cas9 protein and gRNA leads to fast and reliable CRISPR-Cas9 genome editing. 

Fast and reliable gene editing efficiency obtained with jetCRISPR™ in HEK-293 cells (click to enlarge) Fast and reliable gene editing efficiency obtained with jetCRISPR™ in HEK-293 cells (click to enlarge)

Ready-to-use RNP transfection solution

jetCRISPR™ is provided as a ready to use transfection solution: just add the solution to your complexes and then add your cell suspension. Both reverse and forward transfection protocols are available.

CRISPR-Cas9 genome editing has never been easier!

 
Request your jetCRISPR sample here
  •  

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the PolyPlus website to find your local distributor.
Cat no. Description
PO 502-01 jetCRISPR RNP transfection reagent, 0.1 mL
PO 502-07 jetCRISPR RNP transfection reagent, 0.75 mL
PO 502-15 jetCRISPR RNP transfection reagent, 1.5 mL
PO 722-100 SpCas9 Nuclease, 100 µg