jetCRISPR RNP transfection reagent
- RNP transfection reagent for CRISPR-Cas9 genome editing
- Specifically designed for Cas9 protein and guide RNA delivery
- High genome editing efficiency in a wide variety of cell types
- Minimized off-target effects
- Excellent cell viability and morphology
- Compatible with serum and antibiotics
CRISPR/Cas9 is a powerful and highly specific genome editing tool. Choosing the correct method to deliver both Cas9 and guide RNA to your target cells is critical for CRISPR-Cas9 genome editing, as inadequate expression of either component will result in failed genome editing.
jetCRISPR is a RiboNucleoProtein (RNP) transfection reagent that has been specifically designed for efficient co-delivery of Cas9 protein and guide RNA in a wide variety of target cell types.
High CRISPR-Cas9 genome editing efficiency
jetCRISPR™ has been specifically designed for high transfection efficiency of RNP, leading to a high cas9-mediated genome editing efficiency in a variety of cell types.
With jetCRISPR™ you can obtain higher CRISPR-Cas9 genome editing efficiency in comparison with Lipofectamine® CRISPRMAX™.
Combine jetCRISPR with our optimized Cas9 protein, SpCas9 Nuclease, to improve your CRISPR-Cas9 genome editing efficiency even further. SpCas9 Nuclease is a recombinant S. pyogenes Cas9 nuclease that has been optimized to improve nuclear entry following jetCRISPR™-mediated intracellular delivery. The sequence of spCas9 nulcease contains a single SV40 nuclear localization sequence (NLS) in N- and C-terminus.
Easy and reliable CRISPR-Cas9 genome editing
Excellent cell viability and morphology after jetCRISPR RNP transfection
The jetCRISPR RNP transfection reagent is extremely gentle to cells, maintaining an excellent cell viability and morphology.The CRISPR-Cas9 reagent is compatible with serum and antibiotics.
Fast and reliable CRISPR-Cas9 genome editing
Transfection of Cas9 protein instead of Cas9 plasmid allows for faster genome editing: the protein is readily available for use. The Cas9 protein will be cleared from the cells faster than the plasmid, hence minimizing off-target effects and leading to more specific genome editing (Kim S, et al; Genome Research 2014; 24:1012–1019).
Ready-to-use RNP transfection solution
jetCRISPR® is provided as a ready to use transfection solution: just add the solution to your complexes and then add your cell suspension.