jetCRISPR RNP transfection reagent

  • RNP transfection reagent for CRISPR-Cas9 genome editing
  • Specifically designed for Cas9 protein and guide RNA delivery
  • High genome editing efficiency in a wide variety of cell types
  • Minimized off-target effects
  • Excellent cell viability and morphology
  • Compatible with serum and antibiotics

CRISPR/Cas9 is a powerful and highly specific genome editing tool. Choosing the correct method to deliver both Cas9 and guide RNA to your target cells is critical for CRISPR-Cas9 genome editing, as inadequate expression of either component will result in failed genome editing.

jetCRISPR is a RiboNucleoProtein (RNP) transfection reagent that has been specifically designed for efficient co-delivery of Cas9 protein and guide RNA in a wide variety of target cell types.

High CRISPR-Cas9 genome editing efficiency

jetCRISPR™ has been specifically designed for high transfection efficiency of RNP, leading to a high cas9-mediated genome editing efficiency in a variety of cell types.

With jetCRISPR™ you can obtain higher CRISPR-Cas9 genome editing efficiency in comparison with Lipofectamine® CRISPRMAX™.

Superior genome editing efficiency with jetCRISPR™. Click to enlarge. Superior genome editing efficiency with jetCRISPR™. Click to enlarge.

Combine jetCRISPR with our optimized Cas9 protein, SpCas9 Nuclease, to improve your CRISPR-Cas9 genome editing efficiency even further. SpCas9 Nuclease is a recombinant S. pyogenes Cas9 nuclease that has been optimized to improve nuclear entry following jetCRISPR™-mediated intracellular delivery. The sequence of spCas9 nulcease contains a single SV40 nuclear localization sequence (NLS) in N- and C-terminus.

Higher CRISPR-Cas9 genome editing with combined SpCas9 Nuclease and jetCRISPR. Click to enlarge. Higher CRISPR-Cas9 genome editing with combined SpCas9 Nuclease and jetCRISPR. Click to enlarge.

Easy and reliable CRISPR-Cas9 genome editing

Excellent cell viability and morphology after jetCRISPR RNP transfection

The jetCRISPR RNP transfection reagent is extremely gentle to cells, maintaining an excellent cell viability and morphology.The CRISPR-Cas9 reagent is compatible with serum and antibiotics.

Fast and reliable CRISPR-Cas9 genome editing

Transfection of Cas9 protein instead of Cas9 plasmid allows for faster genome editing: the protein is readily available for use. The Cas9 protein will be cleared from the cells faster than the plasmid, hence minimizing off-target effects and leading to more specific genome editing (Kim S, et al; Genome Research 2014; 24:1012–1019).

Ready-to-use RNP transfection solution

jetCRISPR™ is provided as a ready to use transfection solution: just add the solution to your complexes and then add your cell suspension.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the PolyPlus website to find your local distributor.
Cat no. Description
PO 502-01 jetCRISPR RNP transfection reagent, 0.1 mL
PO 502-07 jetCRISPR RNP transfection reagent, 0.75 mL
PO 502-15 jetCRISPR RNP transfection reagent, 1.5 mL
PO 722-100 SpCas9 Nuclease, 100 µg