Xfect Transfection Reagents

  • High transfection efficiency and very low cytotoxicity
  • Simple serum-compatible protocol
  • Suitable for easy and hard-to-transfect cell lines
  • Optimized reagents for stem cells
Xfect is a novel family of transfection reagents based on biodegradable nanoparticle technology. These nanoparticles have a lower cytotoxicity than commonly used reagents and transfects with excellent efficiencies. The Xfect protocol requires few steps and minimal optimization and cells can be transfected in the presence of serum. For DNA transfection three optimized Xfect variants are available.

Xfect Adult Stem Cell
Two of the most commonly used human adult stem cells in research, mesenchymal stem cells (hMSCs) and adipose-derived stem cells (hADSCs) have proven to be difficult-to-transfect with commonly used transfection reagents . Using Xfect Adult Stem Cell Transfection Reagent they can now be transfected with high efficiency and viability.
- Transfects human mesenchymal stem cells (hMSCs) with up to 67% transfection efficiency
- Transfects human adipose-derived stem cells (hADSCs) with up to 99% transfection efficiency
Xfect Adult Stem Cell reagent does not affect the ability of the cells to differentiate.

Xfect mESC
Xfect mESC is a transfection reagent optimized for mouse embryonic stem cells (mESC). Xfect mESC provides both high efficiency and high viability for these cells. In a head-to-head comparison with three other transfection reagents from leading competitors, Xfect mESC provided the best transfection efficiency in multiple mES cell lines, including ES-E14TG2a and ES-D3 mES cell lines.

The basic Xfect reagent can be used for transfection of both easy- and hard-to-transfect cell lines. With commonly used cell lines HeLa, HEK293 and CHO you can achieve efficiencies of 93%, 85% and 83%, respectively.
Xfect's transfection efficiency was also tested on Jurkat cells, which are notorious for their low transfection efficiency. Xfect had > 40-fold higher transfection than competitor product LX.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Clontech website to find your local distributor.
Cat no. Description
CL 631317 Xfect Transfection Reagent - 100 transfections
CL 631318 Xfect Transfection reagent - 300 transfections
CL 631320 Xfect mESC Transfection Reagent - 100 transfections
CL 631321 Xfect mESC Transfection Reagent - 300 transfections
CL 631329 Xfect Transfection reagent for Adult Stem Cells - 100 transfections