DNA Ligation kits

  • Mighty Mix is a one-step master mix containing all ligation components
  • Highly suitable for ligation of sticky, blunt and TA cloning
  • Five-minute ligations are possible
The Mighty Mix Fast Ligation Kit is composed of a single solution containing all required ligation reagents (i.e. enzyme and Buffer). All types of ligation can be performed by simply mixing the Ligation Mix and your DNA. This reaction mix can then be directly used for transformations or for in vitro packaging. The Ligation Mix is stable for up to 50 freeze-thaw cycles.

Good efficiency is obtained not only with sticky-end DNA but also in cloning of blunt-end DNA or in TA cloning where it is sometimes difficult to get higher efficiency.

A 5-minute ligation at 25℃ offers the same efficiency as a 30 min ligation at 16℃for vector ligations, thus saving time.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB 6021 DNA Ligation kit 1.0 - 50 ligations
TB 6022 DNA Ligation Kit 2.1 - 50 ligations
TB 6023 DNA Ligation Kit Mighty Mix, 5 x 150µl
TB 6024 TaKaRa DNA Ligation Kit LONG - 50 ligations