T3 DNA Ligase

T3 DNA Ligase catalyzes the formation of a phosphodiester bond between a 5′ phosphate and a 3′ hydroxyl terminus in double-stranded DNA.

The T3 DNA Ligase enzyme will ligate both sticky and blunt end termini, as well as repair single stranded nicks in dsDNA. Without the addition of 20-30% PEG 6000, T3 DNA Ligase has a very low blunt-end ligation efficiency. T3 DNA Ligase displays a higher ligation efficiency of A/T overhangs compared to C/G overhangs. 

The enzyme is tolarant of moderate salt concentrations: T3 DNA Ligase retains 95% of its activity in 1.0 M NaCl or KCl, with an optimal concentration of 300 mM.

Bulk T3 DNA Ligase for NGS workflows

Need larger quantities of T3 DNA Ligase for your NGS protocols? Contact us for further information and a price quote.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ L6010L T3 DNA Ligase 900000 units