DNA Blunting Kit

Takara’s DNA Blunting Kit transforms 3’ and 5’ overhangs to blunt or flush ends. This transformation is accomplished by the 3’->5’ exonuclease and 5’->3’ polymerase activities of T4 DNA Polymerase. Blunt-ended DNA obtained by this kit can be efficiently ligated into a vector.

Please note that the DNA Blunting kit can blunt DNA fragments with dephosphorylated 5'-protruding ends, but not those with phosphorylated 3'-recessed ends which are often present in sonicated DNA used in shotgun cloning experiments.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB 6025 DNA Blunting Kit, 20rxns