Klenow Fragment DNA Polymerase (Takara)

Klenow Fragment DNA Polymerase I requires template DNA and primer for its function, it selectively catalyzes the transfer of dNTPs to the 3'-OH terminus of the primer that is complementary to the template. This enzyme is purified from E. coli cells and has 3' → 5' exonuclease activity but not 5' 3' exonuclease activity.

Klenow Fragment DNA Polymerase I is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% glycerol.

Klenow Fragment DNA Polymerase I for sequencing is supplied in a buffer of 50 mM potassium phosphate (pH 6.5), 1 mmDTT and 50% ethylene glycol. Ethyleneglycol is contained instead of glycerol in the composition so that good result, without distortion, can be obtained in the sequencing gel electrophoresis.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB 2140A Klenow Fragment (Large Fragment E. coli DNA PolymeraseI) 200u
TB 2140AK Klenow Fragment (Large Fragment E. coli DNA PolymeraseI 2u/?l), 200u