Phi29 DNA Polymerase

  • Huge DNA yields
  • Accurate isothermal amplification
  • Highest processivity DNA polymerase with strong strand displacement activity
  • Exceptionally pure phi29 DNA Polymerase for demanding applications

biotechrabbit™ phi29 DNA Polymerase is an exceptionally pure DNA polymerase for demanding applications. The enzyme is purified from a recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.

The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand-displacement activity and a 3'→ 5' proofreading exonuclease function.

phi29 DNA Polymerase proofreading activity acts preferentially on single-stranded DNA or RNA. Therefore, to avoid primer degradation during the DNA synthesis, 3' modified (protected) primers are highly recommended.

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the biotechrabbit website to find your local distributor.
Cat no. Description
BR BR1100101 phi29 DNA Polymerase (250 U)
BR BR1100102 phi29 DNA Polymerase (1000 U)