Phi29 DNA Polymerase
- Huge DNA yields
- Accurate isothermal amplification
- Highest processivity DNA polymerase with strong strand displacement activity
- Exceptionally pure phi29 DNA Polymerase for demanding applications
biotechrabbit™ phi29 DNA Polymerase is an exceptionally pure DNA polymerase for demanding applications. The enzyme is purified from a recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand-displacement activity and a 3'→ 5' proofreading exonuclease function.
phi29 DNA Polymerase proofreading activity acts preferentially on single-stranded DNA or RNA. Therefore, to avoid primer degradation during the DNA synthesis, 3' modified (protected) primers are highly recommended.