Phi29 DNA Polymerase

  • High-performance Phi29 DNA Polymerase
  • High purity: no detectable ds-endonuclease or E. coli DNA contamination
  • Economical choice for high-volume users

Phi29 DNA Polymerase Unit Definition and Assay

1 unit of Phi29 polymerase is defined as the amount required to convert 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. Activity was assayed using 2-fold serial dilutions of enzyme in reaction buffer. These were added to 50 μL reactions containing λ Hind III DNA, 1X Phi29 DNA Polymerase Reaction Buffer, 3H-dTTP, 0.2 μM dTTP and 200 μM dATP, dCTP, dGTP. Reactions were incubated for exactly 10 minutes at 30°C, put on ice and analyzed using the method of Sambrook and Russell (Molecular Cloning, v3, 2001, pp. A8.25-A8.26).

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Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ P7020-HC-L phi29 DNA Polymerase 2000 units (100,000 U/ml)