T7 DNA Polymerase
T7 DNA Polymerase Product Description
T7 DNA Polymerase is the mesophilic, highly processive, replicative DNA polymerase from bacteriophage T7 that is responsible for the rapid and accurate replication of the virus’ genome during its infection cycle. T7 DNA Polymerase is a two subunit protein, consisting of a polymerase domain (gene 5 from the T7 bacteriophage) and a processivity factor (E. coli trxA gene thioredoxin) (1,2). The enzyme possesses a powerful (3’→5′) nuclease activity that acts on both single and double stranded DNA and appears to be responsible for the high fidelity of this enzyme and prevents strand displacement synthesis (3,4,5).
Source of Protein
A recombinant E. coli strain carrying the bacteriophage T7 gene 5.
50 mM KPO4
0.1 mM EDTA
1.0 mM DTT
pH 7.0 @ 25°C
B7260 (10X T7 DNA Polymerase Buffer)
10X T7 DNA Polymerase Buffer (B7260)
400 mM Tris-HCl
200 mM MgCl2
500 mM NaCl
pH 7.5 @ 25°C