Fpg enzyme

E. coli Fpg protein

Fpg enzyme participates in the base-excision (BER) pathway of DNA repair enzymes and acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity cleaves damaged purines from dsDNA, which creates an apurinic/apyrimidinic (AP site). The AP-lyase activity cleaves both the 3′ and 5′ phosphodiester bonds at the AP site, leaving a single-base gap in the DNA and 3′ and 5′ phosphate termini. Bases recognized and removed by Fpg include 7, 8-dihydro-8- oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapyguanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.

Fpg protein is also known as Formamidopyrimidine DNA glycosylase, Mut M, FAPY DNA Glycosylase, or 8-oxoguanine DNA glycosylase.

 

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Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ Y9070L E. coli Fpg 4000 units (8,000 U/ml)