Micrococcal Nuclease

  • Preferential digestion of ssDNA or ssRNA
  • Also active on double-stranded nucleic acids
  • Completely inactivated by EDTA or EGTA
Micrococcal Nuclease is an endonuclease that preferentially digests single-stranded DNA or RNA at AT or AU-rich regions. Double-stranded DNA or RNA will also be digested. Micrococcal Nuclease yields 3’-phosphate mononucleotides and oligonucleotides due to digestion of 5’-phosphodiester bonds of DNA and RNA.

Key applications:
- Digestion of nucleic acids in crude cell extracts or protein preps
- RNA Sequencing
- Preparation of rabbit reticulocytes lysates

The enzyme requires calcium as a cofactor for its activity and is completely inactivated by EDTA or EGTA.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Takara website to find your local distributor.
Cat no. Description
TB 2910A Micrococcal Nuclease, 15000u