Shrimp Alkaline Phosphatase (SAP), recombinant
- Quickly and easily remove 5' phosphates
- Active on DNA, RNA, dNTPs and proteins
- Completely inactivated after 5 min at 65°C
- Active in common restriction enzyme and PCR buffers
- Excellent stability at 4°C and room temperature
Applications of Shrimp Alkaline Phosphatase
- Removal of nucleotides from PCR prior to sequencing
- Dephosphorylation of restriction-digested cloning vectors to prevent self-ligation
- Rapid dephosphorylation of RNA, proteins or other biomolecules
biotechrabbit™ Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that dephosphorylates most biomolecules and is fully inactivated by heating for 5 min at 65°C.
Prevent self-ligation of cloning vectors
Shrimp Alkaline Phosphatase is an efficient way to reduce self-ligation of digested cloning vectors . The enzyme dephosphorylates all types of DNA termini - 3'-protruding, blunt and 5'-protruding - and is either added to the restriction mixture or after digestion. Shrimp Alkaline Phosphatase can then be efficiently heat-inactivated.
PCR cleanup before sequencing
The combination of Exonuclease 1 (not included) and Shrimp Alkaline Phosphatase provides a simple and fast method for PCR cleanup. Both enzymes are simply added to the PCR mixture, incubated and heat-inactivated. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, while Exonuclease 1 degrades primers, leaving a PCR product that can be directly used for sequencing or genotyping.
If this is your application choose the GenUP ExoSAP Kit, which includes both SAP and Exonuclease I.