Shrimp Alkaline Phosphatase (SAP), recombinant

  • Quickly and easily remove 5' phosphates
  • Active on DNA, RNA, dNTPs and proteins
  • Completely inactivated after 5 min at 65°C
  • Active in common restriction enzyme and PCR buffers
  • Excellent stability at 4°C and room temperature

Applications of Shrimp Alkaline Phosphatase

  • Removal of nucleotides from PCR prior to sequencing
  • Dephosphorylation of restriction-digested cloning vectors to prevent self-ligation
  • Rapid dephosphorylation of RNA, proteins or other biomolecules

biotechrabbit™ Shrimp Alkaline Phosphatase is a multipurpose alkaline phosphatase that dephosphorylates most biomolecules and is fully inactivated by heating for 5 min at 65°C.

Prevent self-ligation of cloning vectors

Shrimp Alkaline Phosphatase is an efficient way to reduce self-ligation of digested cloning vectors . The enzyme dephosphorylates all types of DNA termini - 3'-protruding, blunt and 5'-protruding - and is either added to the restriction mixture or after digestion. Shrimp Alkaline Phosphatase can then be efficiently heat-inactivated.

PCR cleanup before sequencing

The combination of Exonuclease 1 (not included) and Shrimp Alkaline Phosphatase provides a simple and fast method for PCR cleanup. Both enzymes are simply added to the PCR mixture, incubated and heat-inactivated. Shrimp Alkaline Phosphatase dephosphorylates nucleotides and primers, while Exonuclease 1 degrades primers, leaving a PCR product that can be directly used for sequencing or genotyping.

If this is your application choose the GenUP ExoSAP Kit, which includes both SAP and Exonuclease I.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the biotechrabbit website to find your local distributor.
Cat no. Description
BR BR1100601 Shrimp Alkaline Phosphatase, rSAP (500 U)
BR BR1100602 Shrimp Alkaline Phosphatase, rSAP (2500 U)