RecA protein for DNA recombination and repair
RecA protein has its function in DNA recombination and DNA repair. RecA binds to ssDNA, which leads to the polymerization into a nucleoprotein complex. This complex can align with the complementary dsDNA, resulting in DNA strand exchange catalyzed by the RecA enzyme activity.
RecA complexed with site-specific oligos may be used to target and specifically cleave DNA fragments. In addition, a RecA-ATP-single-strand-DNA complex can also function as a cofactor in the proteolytic cleavage of UmuD, LexA, and certain phage proteins.
Source of RecA
Isolated from a recombinant E. coli strain overexpressing E. coli recA from a plasmid.