Uracil DNA Glycosylase (UNG), heat-labile
- Eliminate PCR carryover contamination
- Recombinant purity and consistency
Uracil-DNA Glycosylase (UDG or UNG) is used to prevent PCR amplification of of PCR products carried over from previous reactions.
UNG acts on uracil-containing DNA releasing free uracil. UDG is active on single-stranded or double-stranded DNA, but not on oligomers (less than 7 bases). The removal of uracil fragments the DNA and prevents PCR amplification.
Heat-labile Uracil DNA Glycosylase from cod liver
The enzyme is produced in an E. coli ung-deficient strain expressing a recombinant clone of Gadus morhua (cod) liver UNG. Cod UNG is active at low temperatures and very sensitive to heating. In contrast to other UNGs that can reactivate, Cod UNG is irreversibly inactivated at moderate temperatures. In addition, this enzyme has very high activity in conditions normally used in PCR reactions.