Clontech PCR-Select cDNA Subtraction Kits

  • Ideal for isolating novel, differentially expressed genes
  • Provides greater than 1,000-fold enrichment of rare transcripts
  • Requires only 2 µg of poly A+ RNA
  • cDNA subtraction process takes only 3–4 days
The Clontech PCR-Select™ cDNA Subtraction Kit offers an efficient method for selectively amplifying differentially expressed genes—those genes expressed in one mRNA population but reduced or absent in another. This method is particularly well-suited for the identification of target cDNAs that correspond to rare transcripts, typically the most difficult to obtain. In contrast to other methods that require physically separating single-stranded and double-stranded cDNAs, the Clontech PCR-Select™ method allows the exponential amplification of only the desired sequences.

Subtraction can be performed with just 2 µg of poly A+ RNA. This feature is especially useful when working with RNA samples that are difficult to obtain. If you have only nanograms of total RNA, generate high-quality cDNA for use in Clontech PCR-Select cDNA subtraction with the Super SMART™ PCR cDNA Synthesis Kit.

Advantage® 2 Polymerase Mix is recommended for this kit but not included.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Clontech website to find your local distributor.
Cat no. Description
CL 637401 Clontech PCR-Select cDNA Subtraction Kit
CL 637402 Clontech PCR-Select Diff. Screening Block Sol 1 ml
CL 637403 Clontech PCR-Select Differential Screening Kit