In-Fusion HD PCR Cloning Plus Systems
- No more subcloning: clone directly into any location in any vector
- >95% success rate with fragments of 0.5 to 15 kb
- Assemble up to 5 PCR inserts in one reaction
- No extra unwanted base pairs
- Also suitable for site-directed mutagenesis
Clone any PCR fragment into any linearized vector in a single 15-minute reaction, with In-Fusion Cloning Plus Systems Kits. Inserts are always in the right oriëntation and the procedure introduces no extra unwanted bases. In most cases more than 95% of the clones is correct, which saves you a lot of time verifying clones.
Cloning of multiple fragments in one reaction
The In-Fusion HD Cloning System can efficiently join even multiple fragments in a single reaction. Up to four 1-kb fragments can be assembled simultaneously, just as easy as cloning a single fragment. Cloning of five PCR inserts has even been succesfully tested into one vector. This saves you weeks that you would otherwise spend screening clones and subcloning.
In-Fusion for site-directed DNA mutagenesis
In-Fusion Cloning Kits are not just for cloning: they are also well suited for site-directed DNA mutagenesis. You can add, delete, or replace DNA segments; insert point mutations; insert restriction sites in a single streamlined reaction.
Customer feedback about In-Fusion Cloning