- Degrades RNA from RNA/DNA hybrids
- Inactive against single or double-stranded RNA molecules
RNA degradation from RNA/DNA molecules with RNAse H
RNAse H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules. RNase H digestion produces ribonucleotide molecules with 5- phosphate and 3-hydroxyl termini. The enzyme is nearly inactive against single or double-stranded RNA molecules.
The enzyme is supplied in a buffer containing 20 mM Tris-HCl, 100 mM KCl, 10 mM MgCl2, 0.1 mM EDTA, 0.1 mM DTT and 50% glycerol.