innuCONVERT Bisulfite Conversion Kits
- Complete bisulfite conversion in 45 minutes
- Easy single-tube denaturation and conversion
- Fast DNA cleanup and desulfonation
- High yield of pure, high-quality DNA
- Can be automated
Fast, easy and complete conversion with bisulfite
With the innuCONVERT bisulfite conversion kits you can completely convert non-methylated cytosines to uracil in just a few hours. To simplify the protocol DNA sample denaturation and bisulfite treatment can be done in the same tube.
After a conversion reaction time of only 45 minutes, the converted DNA is isolated and desulfonated on a column, which takes another 45 minutes. The eluted DNA is highly pure, shows little or no degradation and is ready for use in downstream methylation analysis applications such as PCR or sequencing. You don't need specialized equipment: the method is compatible with standard thermocylers or heating blocks.
Choose the optimal conversion kit for your sample type
|Sample type||InnuCONVERT kit|
|Isolated DNA (500 pg-10 µg)||Basic kit|
|Cells (5 x 105)||All-in-One kit|
|Fresh tissue (1 mg)||All-in-One kit|
|FFPE sections (1-3) or FFPE punch biopsies (10 mg)||All-in-One kit|
|Bronchial aspirates, swabs, sputum, urine sediment||All-in-One kit|
|Plasma, serum (3 ml)||Body Fluids kit|
|Urine, ascites, culture sups (3 ml)||Body Fluids kit|
In addition to manual kits we offer the innuCONVERT Bisulfite Purification Kit - IPC16, which allows you to do automated bisulfite conversion and cleanup of 16 DNA samples in parallel on an InnuPure C16 system. The whole process takes less than 90 minutes with very little hands-on time.