EMSA Oligos and Buffer kits
- Easy access and disposal
- Dye is stable for a long time
- Gel (glass plates) can be easily imaged on the Odyssey
- Gel can be replaced back and run longer
Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
Use of IRDye® infrared dyes end-labeled oligonucleotides for EMSA brings along some advantages over use of radioisotopes:
- Infrared assays can be completed in less than two hours with no gel transfer or film exposure.
- The gel doesn’t have to be removed from the glass plates for imaging.
- The gel can be placed back into the electrophoresis unit and run longer, if necessary.