EMSA Oligos and Buffer kits

  • Easy access and disposal
  • Dye is stable for a long time
  • Non-hazardous
  • Gel (glass plates) can be easily imaged on the Odyssey
  • Gel can be replaced back and run longer

Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.

Use of IRDye® infrared dyes end-labeled oligonucleotides for EMSA brings along some advantages over use of radioisotopes:

  • Infrared assays can be completed in less than two hours with no gel transfer or film exposure.
  • The gel doesn’t have to be removed from the glass plates for imaging.
  • The gel can be placed back into the electrophoresis unit and run longer, if necessary.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the LI-COR website to find your local distributor.
Cat no. Description
LI 829-07910 Odyssey EMSA Buffer Kit
LI 829-07921 IRDye 700 p53 Oligos
LI 829-07922 IRDye 700 Stat3 Oligos
LI 829-07923 CREB IRDye 700 Oligos
LI 829-07924 NFkB IRDye 700 Oligos
LI 829-07925 AP-1 IRDye 700 Oligos
LI 829-07926 sp-1 IRDye 700 Oligos
LI 829-07929 IRDye 700 HIF-1 Oligos
LI 927-10100 10X Orange Loading Dye