human BRAF mutation detection primers (V600E, V600K)

  • qPCR Primers for Detection of Human BRAF
  • High sensitivity for mismatch discrimination
  • High confidence: exclusive, definite yes/no answer
  • Simple – no need to use a delta Ct method
  • Performs well with FFPE or fresh frozen samples

The BRAF gene is also known as the proto-oncogene B-Raf and encodes for the protein B-Raf. This protein is involved in the direction of cell growth. A mutation in the BRAF gene is associated with human cancers; most of them are melanomas.

Two-step mutation detection

With the human BRAF mutation detection primers you can detect human BRAF mutations in FFPE or freshly frozen samples. The mutation detection consists of two steps:

Locus-specific qPCR:

A non-allele-specific qPCR is performed to assess total (mutant + wild-type) amplifiable BRAF for each sample. This determines the quantity of DNA to be used in step 2 for each sample.

Allele-specific qPCR

A mutant allele-specific BRAF qPCR is then performed to assess presence of V600E or V600K.

The contents of this kit are sufficient for a total of 60 reactions consisting of 30 locus-specific and 30 allele-specific reactions.

Ultra low copy set for even better detection

An ultra low copy primer set is alaos available for ultra-low copy detection of V600E/K from genomic DNA. This set can detect a single mutant BRAF copy in > 104 wild-type BRAF copies. Regardless of the sensitivity level of mutant BRAF detection needed, myT Primer reagents offer increased specificity, which provides higher confidence in test performance.

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Swift Biosciences website to find your local distributor.
Cat no. Description
SW BRAF-S1 human BRAF mutation detection primers
SW BRAF-U1 human BRAF mutation detection primers-Ultra low copy