CRISPR/Cas9 sgRNA Vector Systems

  • Easy cloning and expression of sgRNAs
  • Co-express Cas9, sgRNA & fluorescent protein marker from a single vector

Guide-it CRISPR/Cas9 Vector Systems allow easy cloning and expression of target single guide RNAs (sgRNAs) for mammalian CRISPR/Cas9 based genome editing.

Express all components from one vector

The pGuide-it vector can simultaneously express Cas9 nuclease, a target-specific sgRNA, and an bright fluorescent protein (ZsGreen1 or tdTomato). Cas9 and the fluorescent protein are co-expressed from a bidirectional CMV promoter, and a user-defined sgRNA is cloned downstream of a human U6 promoter.

The fluorescent protein marker is ideal for monitoring transfection efficiency and/or further enriching/isolating transfected cells by FACS.

Easy cloning

Guide-It plasmid construction is simple. First you design a complementary pair of oligos specific to your target sequence and anneal them to form a duplex. The duplexed oligos are the inserted into the supplied pre-linearized vector using the included high-efficiency ligation mix and Stellar competent cells to ensure high efficiency transformation.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Clontech website to find your local distributor.
Cat no. Description
CL 632601 Guide-it CRISPR/Cas9 System (Green)
CL 632602 Guide-it CRISPR/Cas9 System (Red)