Lentiviral CRISPR/Cas9 Vector System
- Genome editing in hard-to-transfect mammalian cells
- Works for both proliferating and non-proliferating cells
- Single-Shot packaging for easy high-titer lentivirus production
- Prelinearized vector for fast, reliable sgRNA cloning
Lentivirus delivery of sgRNA and Cas9 for genome editing in any mammalian cell type
The Lenti-X CRISPR/Cas9 lentivirus vector system is a complete system for lentiviral-mediated CRISPR/Cas9 genome editing. The system includes Lenti-X Packaging Single Shots for generating high titer lentivirus and two different plasmids for expressing Cas9 and your custom sgRNA in your target cells. By using lentivirus to deliver sgRNA and Cas9, you can achieve targeted genome editing in cell lines that are difficult to transfect.
The plasmid for sgRNA expression, pLVX-hyg-sgRNA1, comes prelinearized for easy insertion of your sgRNA sequence, and the system supplies enough plasmid for you to construct 10 different plasmids each with its own sgRNA sequence.
Knockout efficiency of lentiviral CRISPR/Cas9
Jurkat or HT1080 cells were transduced with LVX-hyg-CD81-sgRNA and selected for stable integration using hygromycin. Stable clones were then transduced with LVX-puro-Cas9. Stable clones were selected for using puromycin and then screened for CD81 knockout efficiency using FACS. Positive and negative controls of parental cells without Cas9 transduction were done either with or without anti-CD81 antibody.