SaCas9 Expression Systems: AAVpro CRISPR/SaCas9

  • Shorter SaCas9 nuclease gene
  • Both SaCas9 and sgRNA fit into one AAV vector
  • Easy, efficient gene editing in a wide variety of mammalian cells

Shorter SaCas9 allows one-construct gene editing

The AAVpro CRISPR/SaCas9 Systems are used for the preparation of an adeno-associated virus (AAV) vector to express the components necessary for CRISPR/SaCas9-mediated genome editing (sgRNA and SaCas9 nuclease) in mammalian cells.

The SaCas9 gene is derived from Staphylococcus aureus and  has a similar editing efficacy to the commonly used Streptococcus pyogenes Cas9 (SpCas9) but is about 1 kb shorter. This makes it is possible to fit both SaCas9 and the sgRNA sequences into a single vector for easier and more efficient genome editing in a wide variety of mammalian cells.

 

SaCas9 Vector Kit formats

The AAVpro CRISPR/SaCas9 Helper Free System (AAV2) (Cat. No. CL 632619) is a complete system, containing reagents to construct custom-designed sgRNA expression plasmids and prepare AAV particles.

The AAVpro CRISPR/SaCas9 Vector System (Cat. No CL 632618) contains the same components except for the packaging system.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Clontech website to find your local distributor.
Cat no. Description
CL 632618 AAVpro CRISPR/SaCas9 Vector System
CL 632619 AAVpro CRISPR/SaCas9 Helper Free System (AAV2)