Guide-it CRISPR/Cas9 sgRNA Screening Kit
Screen to find the best sgRNA for CRISPR/Cas9 editing
The Guide-it sgRNA Screening Kit (Cat. No. 631440) enables the user to test the efficacy of different single guide RNAs (sgRNAs) in vitro prior to using them in studies involving Cas9-mediated gene editing. With this kit, a template containing a sgRNA target site is created by PCR, then combined with an sgRNA that you wish to test and Cas9 nuclease. The efficiency with which Cas9 nuclease cleaves the template can be measured using agarose gel electrophoresis.
How it works