Accel-Amplicon 56G Oncology Panel
- Targets 56 clinically relevant oncology related genes plus Sample_ID
- Illumina sequencer-ready libraries in 2 hours
- Inputs as low as 10 ng DNA, incl. FFPE & cfDNA
- Detection limit as low as 1%
- On-target specificity and coverage uniformity > 95%
Sensitive multiplex NGS of cancer hotspots
The Accel-Amplicon 56G Oncology Panel allows the generation of multiplex oncology-related NGS libraries compatible with Illumina® sequencing platforms. The panel includes 56 clinically-relevant oncology-related genes, amongst which are EGFR, KDR, MAP2K1, PTEN, SMAD4 and many others.
This kit includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters. It is highly suitable for a wide variety of sample types, including FFPE, cfDNA, freshly frozen samples and genomic DNA.
High coverage uniformity for different sample types
10 ng of input DNA from a variety of sample types was used to generate libraries with the Accel-Amplicon 56G Oncology Panel. The coverage uniformity, as the percentage of the bases covered at least 20%, 30%, 40%, or 50% of the average depth, was determined across four sample types. The percentage of reads on target was > 95% for all sample types.
Reproducible Variant Calling from Q-Seq HDx™ Quantitative Standards
The Accel-Amplicon 56G Oncology Panel consistently detected validated variants at the expected frequency in replicates from 10 ng of the Horizon Diagnostics Quantitative Multiplex DNA Reference Standards HD701. The variants were called by LoFreq 2.1.1 (Genome Institute of Singapore) and GATK HaplotypeCaller (Broad Institute). When examining uncommon variants between the 10 replicates, the majority of background variants were present at less than 0.6%. No sporadic variants greater than 0.6% were detected.
Sample_ID as a Spike-in to the 56G Oncology Panel v2
The Accel-Amplicon Sample_ID primers have been manufactured as spiked-in to the 56G Oncology Panel primer pool at a low percentage (2-4% of reads). This enables a sequencing depth of 200X for the germline Sample_ID targets and 5000X for the 56G Oncology targets.
Easy single-tube, 2-hour workflow
The single-tube workflow includes two brief incubations to generate the multiplex amplicon targets and add a unique combination of Illumina®-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.