Accel-NGS DNA 1S Plus Library Prep Kit

  • Generate libraries from degraded, damaged and/or ancient dsDNA or ssDNA
  • Highly efficient adapter ligation
  • Inputs as low as 10 pg
  • Simple & fast, 2-hour protocol
  • High sequence quality and even coverage
  • For Illumina® platforms

The Accel-NGS 1S Plus DNA Library Kit allows you to generate libraries from low input degraded, damaged and/or ancient double-stranded or single-stranded DNA, to be used for Illumina® platforms. This allows:

  • Libraries from ssDNA and dsDNA in a single sample
  • High complexity libraries
  • Fast & simple, 2-hour protocol, start-to-finish
  • Capturing DNA fragments ≥ 40 bp
  • Applicable for various sequencing applications
  • Applicable for a wide range of difficult-to-process samples, including FFPE

Simple 2-hour workflow

Within 2 hours you generate your library DNA for sequencing:

  • The Swift technology adds a low complexity polynucleotide tail with an average length of 8 bases to the 3’ end of each fragment during the addition of the first NGS adapter molecule.
  • The Adaptase Step is a highly efficient, proprietary reaction that simultaneously performs end repair, tailing of 3’ ends, and ligation of the first adapter to 3’ ends.
  • The extension and ligation reactions finish the library molecule by adding the second adapter to 5’ ends.
  • After performing indexing PCR with one of the required indexing kits, your library DNA is ready to go for sequencing analysis!

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Swift Biosciences website to find your local distributor.
Cat no. Description
SW 10024 Accel-NGS 1S Plus DNA Library Kit for Illumina - 24 reactions
SW 10096 Accel-NGS 1S Plus DNA Library Kit for Illumina - 96 reactions
SW 16024 1S Plus Indexing Kit for Illumina - 12 indices, 2 reactions each, Set A
SW 18096 1S Plus Dual Indexing Kit for Illumina - 96 unique combinations