Accel-NGS Methyl-Seq DNA Library Kit

  • Generate high-quality Bisulfite Sequencing libraries
  • Low DNA inputs 100 pg - 100 ng
  • High DNA recovery, low sequence bias
  • Simple, 2-hour protocol
  • For Illumina┬« platform

Excellent Bisulfite Sequencing data from low DNA input

The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite-converted samples and constructs libraries that accurately represent sample composition.

The workflow maximizes DNA recovery through a post-bisulfite library preparation combined with a highly efficient adapter attachment compatible with single-stranded, bisulfite-converted DNA.

Library yields from this kit are up to 100x greater than those from other methods that bisulfite convert after library construction. Additionally, adapter attachment is template-independent, which provides a more complete and less biased library as observed from comprehensive methylome coverage by Whole Genome Bisulfite Sequencing (WGBS).

Superior library complexity and coverage from 1 ng

To demonstrate the efficient and unbiased performance of the Accel-NGS Methyl-Seq Kit, a titration experiment using 100 ng, 10 ng, or 1 ng of Arabidopsis genomic DNA was performed and compared to two alternative methods (random primer and traditional) using these same inputs.

Each Arabidopsis thaliana file was normalized to 30.2 million reads and data reported as an average of duplicate bisulfite-converted samples. To assess coverage for the human genome, an Accel-NGS Methyl-Seq library was constructed using HapMap NA12878 DNA Each Arabidopsis thaliana file was normalized to 30.2 million reads and data reported as an average of duplicate bisulfite-converted samples. To assess coverage for the human genome, an Accel-NGS Methyl-Seq library was constructed using HapMap NA12878 DNA

Methylation Sequencing Applications

  • Whole Genome Bisulfite Sequencing (WGBS)
  • Reduced Representation Bisulfite Sequencing (RRBS)
  • Circulating, cell-free DNA
  • Hybridization capture using NimbleGen™ SeqCap™ Epi Enrichment System 
  • Bisulfite-converted DNA enriched by MeDIP, ChIP or other methods
 
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Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Swift Biosciences website to find your local distributor.
Cat no. Description
SW 30024 Accel-NGS Methyl-Seq DNA Library Kit for Illumina - 24 reactions
SW 30096 Accel-NGS Methyl-Seq DNA Library Kit for Illumina - 96 reactions
SW 36024 Methyl-Seq Set A Indexing Kit (12 indices, 2 reactions each)
SW 38096 Methyl-Seq Dual Indexing Kit for Illumina (96 combinations)