Accel-NGS 2S MID Indexing Kits
- Better detection of low frequency mutations
- Accurate de-duplication
- Differentiate unique fragment reads from PCR duplicates
- Retain more sequencing reads
- Compatible with circulating, cell-free DNA and ChIP-Seq
Low frequency mutation detection
Molecular Identiers, or MIDs improve the detection of low frequency alleles by distinguishing low frequency mutations from synthetic mutations generated by polymerase errors during PCR amplification and sequencing errors.
In addition, these strand-specific MIDs allow accurate filtering of PCR duplicates from fragmentation and strand duplicates. Accurate identification of duplicate reads is especially important when samples have undergone non-random fragmentation.
The Accel-NGS 2S MID Indexing Kits have been optimized and validated for use with the Accel-NGS 2S Plus DNA Library Kit and the Accel-NGS 2S Hyb DNA Library Prep Kit on Illumina platforms. Get the most out of your NGS sequencing data with this powerful tool.
Anatomy of a MID library molecule
The MID adapter carries a strand-specific 9-base random sequence on the P5 adapter (index 2 position). This is paired with a standard low-throughput (LT) P7 adapter, having a 6-base single index (index 1 position) for multiplex sequencing.
Differentiate unique fragment reads from PCR duplicates
Within cfDNA or ChIP libraries, uniquely derived fragments of the same sequence and length may exist in the final dataset. Previously, these unique fragments would be incorrectly identified as PCR duplicates. The only way to differentiate between unique fragment reads and reads from PCR duplicates is by using MIDs.
The use of MIDs in these samples will improve identification of rare somatic mutations in oncology samples, as well as finding transcription factor binding sites in ChIP-Seq samples.
Supported DNA Sequencing Applications
- Whole exome sequencing (WES)
- Deep targeted sequencing
- Cell-free DNA sequencing
- Single-end sequencing, such as ChIP-Seq