TruePrime WGA Kit
- Whole genome amplification without synthetic primers
- No primer artefacts or bias
- Excellent coverage
- High sensitivity as low as 1 fg DNA
- High reproducibility
Primer-free amplification of low input purified DNA
TruePrime™ WGA kit uses a revolutionary novel multiple displacement amplification method based on the combination of the recently discovered DNA primase TthPrimPol and the extremely processive and high-fidelity Phi29 DNA polymerase to amplify uniformly total genomic DNA from purified material. The extraordinary strand displacement capacity of the Phi29 DNA polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification.
Excellent genome coverage
The yeast genome was amplified with TruePrime™ technology and sequenced on an Illumina sequencer. Using 2.5 million read pairs 99.4% of the total yeast genome was covered. The graph shows the evenness of coverage with a narrow distribution curve.