Westburg NGS DNA Library Prep Kit
- Unbiased enzymatic fragmentation
- Highly consistent and tunable fragment size
- Wide input range: 1-1000 ng
- PCR-free for samples >100 ng
- Single-tube, automation-friendly prep
- Excellent coverage uniformity, similar to shearing
Enzymatic fragmentation and library prep in a single tube
The Westburg NGS DNA Library Prep Kit is based on reliable and low-bias enzymatic fragmentation. The simple, straightforward protocol combines DNA fragmentation, end repair and dA-tailing in 1 single step and proceeds with adaptor ligation in the same tube without intermediate cleanup steps.
This workflow reduces library prep time to less than 2.5 hours, with minimal hands-on time and minimal risk of variability. In addition it's a lot easier to automate, compared to mechanical fragmentation methods, such as Covaris shearing.
Highly consistent, tunable fragment size
By simply adjusting the fragmentation time, you can easily fine-tune the fragment size. This way, any desired peak fragment size between 200-600bp can be achieved, to match your specific sequencing applications.
The fragmentation is highly reproducible across different samples, different DNA sources, different GC-percentages and different production lots. This ensures reliable, consistent results.
No GC bias, excellent coverage uniformity
The Westburg NGS DNA Library Prep Kit yields a highly uniform genome coverage, at an average sequencing depth of 25x, delivering sensitive and reliable sequencing data.
Similar to mechanical fragmentation, our enzymatic fragmentation process is sequence-independent and yields consistent coverage across a wide range of GC content. This means that you can be confident that your sequencing data will cover the entire genome, including GC-rich and AT-rich stretches.
The excellent coverage performance delivers reliable and sensitive Whole Genome Sequencing data.
Wide input range, PCR-free for samples >100 ng
Between 1 ng and 1 µg DNA can be used as sample input. The combination of a highly efficient adaptor ligation and having only 1 cleanup step, results in very high library yields. As a consequence, for sample inputs of 100 ng and more library amplification step is not even necessary. This further increases NGS data quality.
In case a library amplification is needed, the Westburg NGS Library Amplification Mix (available separately) provides high-yield, high-fidelity amplification with a very low sequence bias.