The End-Repair mix converts DNA containing damaged or incompatible 5′- and/or 3′-protruding ends to 5′- phosphorylated, blunt-ended DNA. This high-concentration formulation of the End-Repair Mix is compatible with applications requiring >1 microgram of DNA to be prepared for blunt-end ligation.
The conversion to blunt-ended DNA is accomplished by exploiting the 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase. T4 Polynucleotide Kinase ensures that the ends of the blunt-ended DNA fragments are 5′-phosphorylated for subsequent ligation by T4 DNA Ligase.
Bulk End Repair Mix for NGS protocols
Need larger quatities of End Repair Mix for your NGS protocols? Contact us for further information and a price quote.