Klenow Fragment (3'-5' Exo-)

Klenow (3′→5′ exo-) fragment is a mesophilic DNA polymerase deficient in both proofreading (3′→5′) and nick-translation (5′→3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.

Source of Klenow (3'-5' Exo-)

A recombinant E. coli strain carrying the Klenow (3′→5′ exo-) gene.

Specifications

Concentration of Klenow (3'-5' Exo-minus): 50,000 U/mL (HC) or 5,000 U/mL

QC tests:

  • Purity (SDS-PAGE) >99%
  • Specific Activity 10,000 U/mg
  • SS Exonuclease 500 U < 10.0% released
  • DS Exonuclease 500 U < 1.0% released
  • DS Endonuclease 500 U = no conversion
  • E.coli DNA Contamination 500 U < 10 copies
  • UDG Activity < 20 U/mL activity 

Bulk Klenow (3'-5' Exo-) enzyme for NGS applications

Need larger quatities of Klenow fragment (3'-5' Exo-) for your NGS protocols? Contact us for further information and a price quote.

Resources

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ P7010-HC-F Klenow (3’-5’ exo-) 1,540 units (50,000 U/ml)
EZ P7010-HC-L Klenow (3’-5’ exo-) 10,000 units (50,000 U/ml)
EZ P7010-LC-F Klenow (3’-5’ exo-) 1540 units (5,000 U/ml)
EZ P7010-LC-L Klenow (3’-5’ exo-) 10000 units (5,000 U/ml)