T4 Polynucleotide Kinase (PNK)

T4 Polynucleotide Kinase (PNK) catalyzes the transfer and exchange of the terminal gamma position phosphate of ATP to the 5′-hydroxyl terminus of double-and single-stranded DNA, RNA and nucleoside 3′- monophosphate molecules. T4 PNK also exhibits 3′- phosphatase and 2′, 3′ cyclic phosphodiesterase activities.

Source of T4 Polynucleotide Kinase

Purified from a strain of E.coli that expresses the recombinant T4 Polynucleotide Kinase gene. 

Specifications

Concentration: 10,000 units/ml

QC tests:

  • Purity (SDS-PAGE) >99%
  • Specific Activity 133,333 U/mg
  • SS Exonuclease 2000 U <5.0% released
  • DS Exonuclease 2000 U <1.0% released
  • DS Endonuclease 2000 U = No conversion
  • E.coli DNA Contamination 2000 U <10 copies 

Bulk T4 Polynucleotide Kinase for NGS workflows

Need larger quantities of T4 Polynucleotide Kinase for your NGS protocols? Contact us for further information and a price quote.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the MB Enzymes website to find your local distributor.
Cat no. Description
EZ Y9040F T4 Polynucleotide Kinase 1540 units (10,000 U/ml)
EZ Y9040L T4 Polynucleotide Kinase 10000 units (10,000 U/ml)