Swift Normalase Kit
- Enzymatic NGS library quantification and normalisation
- Less effort, less time, higher throughput
- Yields consistent library concentrations
- No qPCR or manual dilutions needed
- Compatible with many NGS workflows
Special offer: 20% Off
Mention promo code SPOT4 on your order to get the discount.
Valid until March 31, 2019; this offer cannot be combined with other discounts or price agreements.
Radically streamline NGS Library normalisation
Is NGS library normalisation slowing you down? Tired of endless qPCRs, calculations and dilutions?
Then check out Swift Normalase, a simple enzymatic normalization procedure that consistently yields NGS libraries with a defined molar concentration. It takes minimal steps and hands-on time and dramatically speeds up your NGS workflow.
The first step of Normalase replaces conventional library amplification, but integrates special primers to pre-condition the libraries, which is followed by two 15-minute incubations to normalize libraries to 4 nM within a single pool.
Fastest, Easiest Workflow
The benefits of Normalase to traditional library quantification methods are that normalization is by molarity vs. by mass, is quantification-free, and does not require individual concentration adjustment or fragment size estimate of each library.
Robust Library Balancing
A. Libraries generated with 1-250 ng DNA were amplified with Normalase primers with the recommended number of PCR cycles to yield ≥12 nM, as confirmed by qPCR.
Libraries were then either normalized with Normalase (B) or manually diluted to 4 nM based on the qPCR quantification and pooled (C): Both pools were sequenced on individual Illumina MiSeq v2 Standard flowcells 1x50 cycles.
B. The Normalase normalized libraries loaded at 12 pM clustered at 892 K/mm2 and the index balance variation was calculated to be a CV of 5.6%.
C. qPCR manually normalized libraries loaded at 10 pM clustered at 848 K/mm2 and the index balance variation was calculated to be a CV of 23%.
Both library pools performed within specifications of MiSeq clustering but Normalase provided 4-fold better library balancing.
Compatible NGS workflows
- Libraries with full-length indexed adapters
- Libraries that have an amplified yield of consistently ≥12nM (20μL volume)
- Libraries prepared for direct sequencing (i.e., whole genome, whole transcriptome)
- Target enriched library pools post-hybridization capture