Swift Normalase Kit
- Enzymatic NGS library quantification and normalisation
- Less effort, less time, higher throughput
- Yields consistent library concentrations
- No qPCR or manual dilutions needed
- Compatible with many NGS workflows
Radically streamline NGS Library normalisation
Is NGS library normalisation slowing you down? Tired of endless qPCRs, calculations and dilutions?
Then check out Swift Normalase, a simple enzymatic normalization procedure that consistently yields NGS libraries with a defined molar concentration. It takes minimal steps and hands-on time and dramatically speeds up your NGS workflow.
The first step of Normalase replaces conventional library amplification, but integrates special primers to pre-condition the libraries, which is followed by two 15-minute incubations to normalize libraries to 4 nM within a single pool.
Fastest, Easiest Workflow
The benefits of Normalase to traditional library quantification methods are that normalization is by molarity vs. by mass, is quantification-free, and does not require individual concentration adjustment or fragment size estimate of each library.
Robust Library Balancing
A. Libraries generated with 1-250 ng DNA were amplified with Normalase primers with the recommended number of PCR cycles to yield ≥12 nM, as confirmed by qPCR.
Libraries were then either normalized with Normalase (B) or manually diluted to 4 nM based on the qPCR quantification and pooled (C): Both pools were sequenced on individual Illumina MiSeq v2 Standard flowcells 1x50 cycles.
B. The Normalase normalized libraries loaded at 12 pM clustered at 892 K/mm2 and the index balance variation was calculated to be a CV of 5.6%.
C. qPCR manually normalized libraries loaded at 10 pM clustered at 848 K/mm2 and the index balance variation was calculated to be a CV of 23%.
Both library pools performed within specifications of MiSeq clustering but Normalase provided 4-fold better library balancing.
Compatible NGS workflows
- Libraries with full-length indexed adapters
- Libraries that have an amplified yield of consistently ≥12nM (20μL volume)
- Libraries prepared for direct sequencing (i.e., whole genome, whole transcriptome)
- Target enriched library pools post-hybridization capture