SMART-Seq V4 Ultra Low Input mRNA-Seq kit

  • Single Cell mRNA-Seq: use only 10 pg total RNA or 1 cell
  • Identify the highest number of genes
  • Great representation of GC-rich transcripts
  • Get full-length gene information
  • Single-tube protocol works directly on cells

Single-Cell mRNA Seq with Ultimate Sensitivity

The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth-generation of ultra-low-input RNA-Seq kits, and our most sensitive mRNA-seq kit for single cells. This new kit combines two powerful technologies: SMART and LNA (locked nucleic acid). The result outperforms both the SMART-seq2 method and previous SMARTer Ultra Low input NGS kits by identifying the greatest number of transcripts, including low expressed and GC-rich genes.

More genes identified & higher genome mapping

Compared to SMARTer Ultra Low v3 kit or SMART-Seq2 a higher percentage of reads from the SMART-Seq v4 kit map to the genome, showing that the improvements decrease background. In addition the SMART-Seq v4 libraries identify significantly more transcripts than the other methods:

Sequencing Metrics Comparing Different cDNA Synthesis Protocols
cDNA synthesis method SMARTer v3 SMART-Seq v4 SMART-Seq2
% mapped to rRNA 0.8 0.4 6.5 6.1 6.9 3.8
% mapped to genome 96 97 96 95 72 93
% mapped to exons 73 73 76 76 66 67
% mapped to introns 21 21 19 20 28 27
# of genes FPKM >0.1 11,647 10,885 14,731 14,813 12,080 12,039
FPKM >1 9,729 9,105 12,501 12,591 10,270 10,058

See all the metrics 

Higher Reproducibility for Genes with Low Expression

For low expressed genes the SMART-Seq v4 kit has a much higher correlation between replicates (Pearson R = 0.739) compared to the SMARTer Ultra Low v3 kit or the SMART-Seq2 method. A higher reproducibility means that you can have more confidence that differences in expression levels between samples are biologically relevant, and are not caused by experimental variation.

Ordering information

Westburg offers this product in The Netherlands, Belgium, Luxembourg,
If you are not located in these countries please visit the Clontech website to find your local distributor.
Cat no. Description
CL 634888 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 12 Rxns
CL 634889 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 24 Rxns
CL 634890 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 48 Rxns
CL 634891 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 96 Rxns
CL 634892 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 192 Rxns
CL 634893 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 480 Rxns
CL 634894 SMART-Seq V4 Ultra Low Input RNA kit for Sequencing 960 Rxns
CL 635013 10X Single Cell Lysis Buffer