Legacy SMARTer Ultra Low mRNA-Seq Kits
- Single-tube protocol for whole cells preserves sample integrity
- Start with 1–1,000 cells or 10 pg–10 ng total RNA
- Compatible with Illumina® and Ion Torrent (V3 only) NGS platforms
Clontech offers multiple generations of ultra low input transcriptome sequencing kits. The core SMART technology in these legacy kits is an immensely powerful tool for transcriptome and full-length transcript analyses, including investigations in splice junctions and alternative splice variants in cells. These kits use oligo(dT) priming and allow for cDNA synthesis directly from whole cells, without any additional sample preparation, or from total RNA of high integrity (RIN 8–10). cDNA libraries can be simply and efficiently generated for transcriptome analysis (mRNA-seq) from single cells, a few to 1,000 intact cells, or 10 pg–10 ng of total RNA.
All legacy SMARTer Ultra Low kits generate libraries that are compatible with Illumina® instruments. The SMARTer Ultra Low Input RNA Kit for Sequencing - v3 has also been tested with Ion Torrent sequencing library preparation kits.
We recommend completing ongoing experiments with the same SMARTer Ultra Low legacy kit that you started with. For new users we recommend the new SMART-Seq V4 Low Input RNA Kit for Sequencing